This study aimed to assess the validity and reliability of the dark personality instrument in students of general physical education units of Mashhad universities. The participants include all students of Ferdowsi, Imam Reza, Islamic Azad, and Payame Noor universities who had chosen the units of general physical education and sport in the academic year of 2021-22, using the Morgan table, 196 people were randomly selected as a sample. A standard dark personality questionnaire (Jonason & Webster, 2010) was used to collect data. Cronbach's alpha test was used to confirm the reliability of the questionnaire. To confirm the instrument's validity, exploratory and confirmatory factor analyses were used. Data analysis showed that the factor load of all items is higher than the baseline value (0.4) and the research model has a significant fit. Also, the model fit indices had acceptable values. Finally, it is recommended to sports coaches and teachers of physical education classes and leisure time to use this scale at the beginning of each semester to get to know more about the personality characteristics of students in their class and to measure these people, this can help them a lot in how to manage their classes.