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Mutational Analysis of Driver and Non-driver Mutations of Philadelphia Chromosome-negative Myeloproliferative Neoplasms; Diagnosis and Recent Advances in Treatment
World Journal of Cancer and Oncology Research
| Vol 3, Issue 1
Table 4. Methods for MPN mutational analysis.Adapted from: [14].
| Method | Sensitivity (%) | Advantage | Disadvantage |
| Sanger sequencing | 20 | All mutations detected; bidirectional confirmation | Poor sensitivity; takes time; not quantitative |
| Restriction Fragment Length polymorphism | 1-10 | Less expensive | Not quantitative; relatively low sensitivity; requires post-PCR modifications; high + samples required for good results |
| Pyrosequencing | 5-10 | Easy to perform; semiquantitative; less expensive | Only target mutations detected; relatively low sensitivity |
| Melt curve analysis | 5-10 | Easy to perform; semiquantitative; less expensive | Only target mutations detected; relatively moderate sensitivity; high + samples required for good results |
| Allele-specific PCR | 0.1-1 | Highly sensitive and easy to perform | Only target mutations detected; not quantitative |
| Locked nuclei acid qPCR | 0.1-0.01 | Highly sensitive; quantitative | Only target mutations detected |
| Allele-specific qPCR | 0.1-0.01 | Highly sensitive; quantitative | Only target mutations detected |