<b>Background:</b> <i>Gallibacterium</i><i> </i><i>anatis</i> is an opportunistic pathogen, mainly associated with infections in poultry. Infections in humans have only been described four times (in France and China). <b>Case presentation:</b> We report the case of a 62-year-old non-immunocompromised male patient with a gastric bypass in his medical history, presenting with cholecystitis in whom <i>Gallibacterium</i><i> </i><i>anatis</i> was isolated from the blood, besides an <i>Escherichia coli</i>. Identification of the <i>G. </i><i>anatis</i> was achieved using MALDI-TOF MS and confirmatory 16s RNA sequencing. The patient was successfully treated with piperacillin-tazobactam, resulting in clinical improvement. In the context of impaired host defenses due to acute cholecystitis with an <i>E. coli</i> bacteremia, it is conceivable that <i>G. </i><i>anatis</i> entered the bloodstream through bacterial translocation from the gastrointestinal tract. <b>Conclusion:</b><b> </b>This case highlights the importance of advanced identification techniques and awareness of rare pathogens in clinical practice. <i>Gallibacterium</i><i> </i><i>anatis</i>, particularly drug-resistant strains, may represent a potential infectious agent and should therefore be considered in diagnostic work-up.